We use the The FERTILE Microfluidic Sperm Sorting Chip is a flow- and chemical-free, single-use device having an inlet sample chamber connected to an outlet collection chamber by a microfluidic channel. An untreated semen sample is pipetted into the inlet chamber and the sorted sperm are collected from the outlet. Sperm are sorted by the separation of healthy motile sperm from the many compromised sperm present in the semen. Sorted sperm exhibit better morphology, lower levels of reactive oxygen species (ROS) and less DNA fragmentation than the original semen sample. Retreatment of the semen sample is not required; thereby, reducing the risk of contamination.